Quantitative morphological signatures define local signaling networks regulating cell morphology.

Supporting Online Material for Local Signaling Networks That Regulate Cell Morphology Defined by Quantitative Morphological Signatures

Quantitative analysis of colony morphology in yeast.

Microorganisms often form multicellular structures such as biofilms and structured colonies that can influence the organism's virulence, drug resistance, and adherence to medical devices. Phenotypic classification of these structures has traditionally relied on qualitative scoring systems that limit detailed phenotypic comparisons between strains. Automated imaging and quantitative analysis hav...

Extending Morphological Signatures for Visual Pattern Recognition

Morphological signatures are powerful descriptions of the image content which are based on the framework of mathematical morphology. These signatures can be computed on a global or local scale: they are called pattern spectra (or granulometries and antigranulometries) when measured on the complete images and morphological profiles when related to single pixels. Their goal is to measure shape di...

Long non-coding RNA FOXO1 inhibits lung cancer cell growth through down-regulating PI3K/AKT signaling pathway

Objective(s): Lung cancer is one of the most common malignant tumors, which seriously threatens the health and life of the people. Recently, a novel long non-coding RNA (lncRNA) termed lncFOXO1 was found and investigated in breast cancer. However, the effect of lncFOXO1 on lung cancer is still ambiguous. The current study aimed to uncover the functions of lncFOXO1 in l...

A high-content analysis toolbox permits dissection of diverse signaling pathways for T lymphocyte polarization.

RNA interfering (RNAi) screening strategies offer the potential to elucidate the signaling pathways that regulate integrin and adhesion receptor-mediated changes in T lymphocyte morphology. Of crucial importance, however, is the definition of key sets of parameters that will provide accurate, quantitative, and nonredundant information to flag relevant hits in such assays. In this study, the aut...